Hydroxylation of diverse flavonoids by CYP450 BM3 variants: biosynthesis of eriodictyol from naringenin in whole cells and its biological activities
Background Cytochrome P450 monooxygenase constitutes a significant group of oxidative
enzymes that can introduce an oxygen atom in a high regio-and stereo-selectivity mode. We
used the Bacillus megaterium cytochrome P450 BM3 (CYP450 BM3) and its variants namely
mutant 13 (M13) and mutant 15 (M15) for the hydroxylation of diverse class of flavonoids.
Results Among 20 flavonoids, maximum seven flavonoids were hydroxylated by the variants
while none of these molecules were accepted by CYP450 BM3 in in vitro reaction …
enzymes that can introduce an oxygen atom in a high regio-and stereo-selectivity mode. We
used the Bacillus megaterium cytochrome P450 BM3 (CYP450 BM3) and its variants namely
mutant 13 (M13) and mutant 15 (M15) for the hydroxylation of diverse class of flavonoids.
Results Among 20 flavonoids, maximum seven flavonoids were hydroxylated by the variants
while none of these molecules were accepted by CYP450 BM3 in in vitro reaction …
Hydroxylation of diverse flavonoids by CYP450 BM3 variants: biosynthesis of eriodictyol from naringenin in whole cells and its biological activities.
CLL Chu LuanLuong, RP Pandey, JNR Jung NaRae… - 2016 - cabidigitallibrary.org
Background: Cytochrome P450 monooxygenase constitutes a significant group of oxidative
enzymes that can introduce an oxygen atom in a high regio-and stereo-selectivity mode. We
used the Bacillus megaterium cytochrome P450 BM3 (CYP450 BM3) and its variants namely
mutant 13 (M13) and mutant 15 (M15) for the hydroxylation of diverse class of flavonoids.
Results: Among 20 flavonoids, maximum seven flavonoids were hydroxylated by the
variants while none of these molecules were accepted by CYP450 BM3 in in vitro reaction …
enzymes that can introduce an oxygen atom in a high regio-and stereo-selectivity mode. We
used the Bacillus megaterium cytochrome P450 BM3 (CYP450 BM3) and its variants namely
mutant 13 (M13) and mutant 15 (M15) for the hydroxylation of diverse class of flavonoids.
Results: Among 20 flavonoids, maximum seven flavonoids were hydroxylated by the
variants while none of these molecules were accepted by CYP450 BM3 in in vitro reaction …
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