Identification and functional characterization of rat riboflavin transporter 2
S Yamamoto, K Inoue, K Ohta, R Fukatsu… - Journal of …, 2009 - academic.oup.com
S Yamamoto, K Inoue, K Ohta, R Fukatsu, J Maeda, Y Yoshida, H Yuasa
Journal of biochemistry, 2009•academic.oup.comWe have newly identified rat riboflavin transporter 2 (rRFT2) and its human orthologue
(hRFT2), and carried out detailed functional characterization of rRFT2. The mRNA of rRFT2
was highly expressed in jejunum and ileum. When transiently expressed in human
embryonic kidney (HEK) 293 cells, rRFT2 could transport riboflavin efficiently. Riboflavin
transport mediated by rRFT2 was Na+-independent but moderately pH-sensitive, being
more efficient in acidic conditions than in neutral and basic conditions. Kinetic analysis …
(hRFT2), and carried out detailed functional characterization of rRFT2. The mRNA of rRFT2
was highly expressed in jejunum and ileum. When transiently expressed in human
embryonic kidney (HEK) 293 cells, rRFT2 could transport riboflavin efficiently. Riboflavin
transport mediated by rRFT2 was Na+-independent but moderately pH-sensitive, being
more efficient in acidic conditions than in neutral and basic conditions. Kinetic analysis …
Abstract
We have newly identified rat riboflavin transporter 2 (rRFT2) and its human orthologue (hRFT2), and carried out detailed functional characterization of rRFT2. The mRNA of rRFT2 was highly expressed in jejunum and ileum. When transiently expressed in human embryonic kidney (HEK) 293 cells, rRFT2 could transport riboflavin efficiently. Riboflavin transport mediated by rRFT2 was Na+-independent but moderately pH-sensitive, being more efficient in acidic conditions than in neutral and basic conditions. Kinetic analysis indicated that rRFT2-mediated riboflavin transport was saturable with a Michaelis constant (Km) of 0.21 μM. Furthermore, it was specifically and strongly inhibited by lumiflavin, flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD), and to a lesser extent by amiloride. Such ability to transport riboflavin in a specific manner, together with its high expression in the small intestine, indicates that RFT2 may play a role in the intestinal absorption of riboflavin.
![](/scholar/images/qa_favicons/oup.com.png)
以上显示的是最相近的搜索结果。 查看全部搜索结果