The objective of this study was to identify random amplified polymorphic DNA (RAPD) markers linked to the stripe rust [Puccinia striiformis] resistance genes in F₂ populations of wheat by using the bulked segregant analysis (BSA) procedure with RAPD markers. F₂ populations have been derived from two crosses: Sakha 61 × Sakha 8 (cross I) and Sakha 61 × Sids 7 (cross II). Sakha 61 contains the stripe rust resistance gene, while, Sakha 8 and Sids 7 are susceptible to that disease. Using BSA in conjunction with RAPD analysis, the 450 bp polymorphic fragment, revealed by Primer 8 (GAAACGGGTG) in the first population, and the 700 bp polymorphic fragment, revealed by Primer 18 (GACCGCTTGT) in the second population, were found to be linked to the stripe rust resistance gene. Analysis of genetic segregation of the polymorphic markers (Primer 8₄₅₀ and Primer 18₇₀₀), tested in the F₂ populations, fitted the expected 3:1 Mendelian ratio. The polymorphic marker (Primer 18₇₀₀) was identified in the monogenic line, Yr₁, but was absent in the other monogenic lines. Based on these results, it was concluded that Primer 18 might prove to be useful in early generation selection for Yr₁.