Identification of novel mutations in genes involved in silicon and arsenic uptake and accumulation in rice
HJ Kim, TH Tai - Euphytica, 2019 - Springer
Novel mutations in rice genes involved in silicon (Si) and arsenic (As) transport (Lsi1, Lsi2)
and vacuolar sequestration of As (OsABCC1) were identified using reverse genetics.
TILLING by sequencing of a population of chemically-induced mutants (n= 2048) detected
61 putative mutations. Following removal of mutations predicted to be synonymous or
residing in introns, Sanger sequencing confirmed 21 of 23 nonsynonymous mutations and
13 M3 lines harboring homozygous mutant alleles (three lsi1, nine lsi2, and one Osabcc1) …
and vacuolar sequestration of As (OsABCC1) were identified using reverse genetics.
TILLING by sequencing of a population of chemically-induced mutants (n= 2048) detected
61 putative mutations. Following removal of mutations predicted to be synonymous or
residing in introns, Sanger sequencing confirmed 21 of 23 nonsynonymous mutations and
13 M3 lines harboring homozygous mutant alleles (three lsi1, nine lsi2, and one Osabcc1) …
Identification of novel mutations in genes involved in silicon and arsenic uptake and accumulation in rice.
KHJ Kim HyunJung, TH Tai - 2019 - cabidigitallibrary.org
Novel mutations in rice genes involved in silicon (Si) and arsenic (As) transport (Lsi1, Lsi2)
and vacuolar sequestration of As (OsABCC1) were identified using reverse genetics.
TILLING by sequencing of a population of chemically-induced mutants (n= 2048) detected
61 putative mutations. Following removal of mutations predicted to be synonymous or
residing in introns, Sanger sequencing confirmed 21 of 23 nonsynonymous mutations and
13 M3 lines harboring homozygous mutant alleles (three lsi1, nine lsi2, and one Osabcc1) …
and vacuolar sequestration of As (OsABCC1) were identified using reverse genetics.
TILLING by sequencing of a population of chemically-induced mutants (n= 2048) detected
61 putative mutations. Following removal of mutations predicted to be synonymous or
residing in introns, Sanger sequencing confirmed 21 of 23 nonsynonymous mutations and
13 M3 lines harboring homozygous mutant alleles (three lsi1, nine lsi2, and one Osabcc1) …
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