Genome sequencing has revealed that fungi have the ability to synthesize many more
natural products (NPs) than are currently known, but methods for obtaining suitable
expression of NPs have been inadequate. We have developed a successful strategy that
bypasses normal regulatory mechanisms. By efficient gene targeting, we have replaced, en
masse, the promoters of nonreducing polyketide synthase (NR-PKS) genes, key genes in
NP biosynthetic pathways, and other genes necessary for NR-PKS product formation or …