Immobilization of sugars in supermacroporous cryogels for the purification of lectins by affinity chromatography

GRF Gonçalves, ORR Gandolfi, LS Santos… - … of Chromatography B, 2017 - Elsevier
Lectins are glycoproteins that bind to carbohydrates or glycoconjugates by specific
interactions. The specificity of lectins to various carbohydrates is a determinant factor in the
choice of ligand for the chromatographic matrix when using chromatography as a lectin
purification technique. In this work, the immobilization of three different aminated
carbohydrates on the surface of macroporous polymeric cryogels was evaluated.
Carbohydrates were immobilized on cryogel surfaces via the glutaraldehyde method to …
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