Both the host microbiome and the microbiome of the built environment can have profound
impacts on human health. While prior studies have suggested that the variability introduced
by DNA extraction method is less than typical biologic variation, most studies have focused
on 16S rRNA amplicon sequencing or on high biomass fecal samples. Shotgun
metagenomic sequencing provides advantages over amplicon sequencing for surveying the
microbiome, but is a challenge to perform in lower microbial biomass samples with high …