Improved specimen reconstruction by Hilbert phase contrast tomography
B Barton, F Joos, RR Schröder - Journal of structural biology, 2008 - Elsevier
The low signal-to-noise ratio (SNR) in images of unstained specimens recorded with
conventional defocus phase contrast makes it difficult to interpret 3D volumes obtained by
electron tomography (ET). The high defocus applied for conventional tilt series generates
some phase contrast but leads to an incomplete transfer of object information. For
tomography of biological weak-phase objects, optimal image contrast and subsequently an
optimized SNR are essential for the reconstruction of details such as macromolecular …
conventional defocus phase contrast makes it difficult to interpret 3D volumes obtained by
electron tomography (ET). The high defocus applied for conventional tilt series generates
some phase contrast but leads to an incomplete transfer of object information. For
tomography of biological weak-phase objects, optimal image contrast and subsequently an
optimized SNR are essential for the reconstruction of details such as macromolecular …