Inhibition of sodium hypochlorite antimicrobial activity in the presence of bovine serum albumin
FG Pappen, W Qian, J Aleksejūnienė… - Journal of …, 2010 - Elsevier
FG Pappen, W Qian, J Aleksejūnienė, R de Toledo Leonardo, MR Leonardo, M Haapasalo
Journal of endodontics, 2010•ElsevierINTRODUCTION: This study investigated the inhibition of the antimicrobial activity of sodium
hypochlorite (NaOCl) by bovine serum albumin (BSA). The killing of Enterococcus faecalis,
Candida albicans, Staphylococcus epidermidis, and Escherichia coli by NaOCl in
concentrations from 2% to 0.03% was measured in the presence of BSA in concentrations
between 6.7% and 0.1%. METHODS: NaOCl, BSA, and microorganism suspensions were
mixed, and, after 30 seconds, 6 minutes, and 30 minutes, samples were taken and NaOCl …
hypochlorite (NaOCl) by bovine serum albumin (BSA). The killing of Enterococcus faecalis,
Candida albicans, Staphylococcus epidermidis, and Escherichia coli by NaOCl in
concentrations from 2% to 0.03% was measured in the presence of BSA in concentrations
between 6.7% and 0.1%. METHODS: NaOCl, BSA, and microorganism suspensions were
mixed, and, after 30 seconds, 6 minutes, and 30 minutes, samples were taken and NaOCl …
INTRODUCTION
This study investigated the inhibition of the antimicrobial activity of sodium hypochlorite (NaOCl) by bovine serum albumin (BSA). The killing of Enterococcus faecalis, Candida albicans, Staphylococcus epidermidis, and Escherichia coli by NaOCl in concentrations from 2% to 0.03% was measured in the presence of BSA in concentrations between 6.7% and 0.1%.
METHODS
NaOCl, BSA, and microorganism suspensions were mixed, and, after 30 seconds, 6 minutes, and 30 minutes, samples were taken and NaOCl was inactivated by 5% sodium thiosulphate. The microbes were incubated in tryptic soy broth broth for up to 7 days for the detection of growth.
RESULTS
All microorganisms were killed within 30 seconds by 0.03% NaOCl when BSA was not present. High concentrations of BSA significantly reduced the antimicrobial activity of NaOCl against the four species.
CONCLUSIONS
The inhibition of sodium hypochlorite by BSA was directly dependent on their quantitative relationships. The result partly explains the poorer performance in vivo of NaOCl as compared to in vitro experiments.
Elsevier
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