[HTML][HTML] Multicenter benchmarking of short and long read wet lab protocols for clinical viral metagenomics

FX Lopez-Labrador, M Huber, IA Sidorov… - Journal of Clinical …, 2024 - Elsevier
… Performance of the metagenomic protocols was analyzed using a validated central pipeline
… Metagenomic inter-laboratory comparisons and EQA schemes using cell-culture based or …

An interlaboratory proficiency test using metagenomic sequencing as a diagnostic tool for the detection of RNA viruses in swine fecal material

L Liu, M Hakhverdyan, P Wallgren… - Microbiology …, 2024 - journals.asm.org
research project METASTAVA (Standardisation and validation of metagenomic methods for
the detection … successful interlaboratory study using high-throughput sequencing methods or …

Metrology and Molecular Diagnosis of Infection

EJ Busby - 2020 - discovery.ucl.ac.uk
… This thesis examines the placing of metrology in molecular diagnosis of infectious diseases.
… assignment of reference materials to harmonise inter-laboratory results. MALDI-TOF MS …

Multi-laboratory comparison of next-generation to sanger-based sequencing for HIV-1 drug resistance genotyping

NT Parkin, S Avila-Rios, DF Bibby, CJ Brumme… - Viruses, 2020 - mdpi.com
… A crucial function of the VQA program is to ensure the validity … In this paper, we report for the
first time an inter-laboratory … We thank the High-throughput Sequencing Facility at University …

Interlaboratory comparison using inactivated SARS-CoV-2 variants as a feasible tool for quality control in COVID-19 wastewater monitoring

A Wilhelm, J Schoth, C Meinert-Berning… - Science of the Total …, 2023 - Elsevier
… ) for all spiked samples with a mean intra- and inter-laboratory variability of 19 % and 104
%, … The high throughput sequencing (HTS) technology enables the study of full-length viral

[HTML][HTML] Clinical establishment of a laboratory developed quantitative HDV PCR assay on the cobas6800 high-throughput system

LS Pflüger, D Nörz, T Volz, K Giersch, A Giese… - JHEP Reports, 2021 - Elsevier
… by considerable run-to-run and inter-laboratory variability. Hence, we established a …
most severe form of viral hepatitis. Studies have shown that high HDV viral loads (>600,000 …

Current impact and future directions of high throughput sequencing in plant virus diagnostics

S Massart, A Olmos, H Jijakli, T Candresse - Virus research, 2014 - Elsevier
… It is now raising a growing interest for virus diagnostics. This review provides a snapshot
vision on the current use and impact of high throughput sequencing approaches in plant virus

Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples

A Pluta, JP Jaworski, C Droscha, S VanderWeele… - … Veterinary Research, 2024 - Springer
high throughput of serological tests, there are several scenarios where highly specific and
sensitive molecular assays for the detection of BLV DNA might improve detectionvalidated by …

[HTML][HTML] Development of a candidate reference material for adventitious virus detection in vaccine and biologicals manufacturing by deep sequencing

ET Mee, MD Preston, PD Minor, S Schepelmann… - Vaccine, 2016 - Elsevier
… or high throughput sequencing) offers the potential for … materials to enable validation and
comparison of methods. … consistency and enabling inter-laboratory comparisons to support this …

High quality of SARS-CoV-2 molecular diagnostics in a diverse laboratory landscape through supported benchmark testing and External Quality Assessment

J Sluimer, WMR van den Akker, G Goderski, A Swart… - Scientific reports, 2024 - nature.com
… ) assays for the detection of the novel SARS-CoV-2 virus 1 . … to start diagnostic testing or
high throughput testing for the … with regular EQA and inter-laboratory comparisons as shown …