Colletotrichum lindemuthianum is the causal agent of anthracnose of common bean (Phaseolus vulgaris) and the genetic resistance is the best method to control the disease. However, the wide virulence diversity of this pathogen has complicated the breeding for resistance. Studies of race characterization using the standard set of 12 differential cultivars (Pastor Corrales, 1991) have shown the predominance of races 65 and 81 in Brazil (Silva et al., 2007). Previously studies showed that genetic variability within race 65 makes difficult the development of a durable resistance (Davide & Souza, 2009; Ishikawa et al., 2011). Common bean lines able to differentiate the variation within race 65 to complement the results of the standard set of differential cultivars and a new nomenclature to identify the pathogenic variability of race 65 strains was proposed by Ishikawa et al (2011). The objective of this work was evaluate if the same common bean lines can be used to identify variability within race 81 of C. lindemuthianum.