[PDF][PDF] Involvement of pyruvate on oxidative stress management in the microaerophilic protozoan parasite Giardia lamblia
Background: Giardia lamblia, a microaerophilic protozoon produces energy by fermentative
metabolism. It is devoid of conventional mechanisms of oxidative stress management,
including superoxide dismutase, catalase, peroxidase, and glutathione cycling, which are
present in most eukaryotes. To protect and establish its pathogenesis within human gut it
has to develop antioxidant defense strategies to grapple with elevated oxygen tensions,
which are harmful for its survival. Methods: Intracellular reactive oxygen species (ROS) …
metabolism. It is devoid of conventional mechanisms of oxidative stress management,
including superoxide dismutase, catalase, peroxidase, and glutathione cycling, which are
present in most eukaryotes. To protect and establish its pathogenesis within human gut it
has to develop antioxidant defense strategies to grapple with elevated oxygen tensions,
which are harmful for its survival. Methods: Intracellular reactive oxygen species (ROS) …
Background: Giardia lamblia, a microaerophilic protozoon produces energy by fermentative metabolism. It is devoid of conventional mechanisms of oxidative stress management, including superoxide dismutase, catalase, peroxidase, and glutathione cycling, which are present in most eukaryotes. To protect and establish its pathogenesis within human gut it has to develop antioxidant defense strategies to grapple with elevated oxygen tensions, which are harmful for its survival.
Methods: Intracellular reactive oxygen species (ROS) generation by Giardia suspension was monitored with the help of a dichlorodihydrofluoresceine diacetate based assay. Linoleic acid micelles were employed to investigate the lipid radical scavenging activity of pyruvate. In this study, we examined the effects of pyruvate addition during oxidative stress on DNA damage in Giardia. The pyruvate concentrations at different time points were measured during oxidative stress condition in
researchgate.net
以上显示的是最相近的搜索结果。 查看全部搜索结果