Purification of nucleic acids using isotachophoresis

A Rogacs, LA Marshall, JG Santiago - Journal of Chromatography A, 2014 - Elsevier
… with those of nucleic acids. We therefore recommend exploring alternate … to purify nucleic
acid samples with humic acid contamination, either instead of or in addition to ITP purification. …

Purification of nucleic acids from whole blood using isotachophoresis

A Persat, LA Marshall, JG Santiago - Analytical chemistry, 2009 - ACS Publications
… for the purification of nucleic acids from biological samples using isotachophoresis (ITP). We
… to achieve ITP-based extraction, preconcentration, and purification of DNA from nanoliter …

[HTML][HTML] On-chip isotachophoresis for separation of ions and purification of nucleic acids

G Garcia-Schwarz, A Rogacs… - Journal of visualized …, 2012 - pmc.ncbi.nlm.nih.gov
purify nucleic acids from untreated cell lysate by selecting a trailing anion with an effective
mobility magnitude lower than the target nucleic acid … and focuses target nucleic acids from the …

Recent progress in nucleic acids isotachophoresis

V Datinská, I Voráčová, U Schlecht… - Journal of Separation …, 2018 - Wiley Online Library
… Since isotachophoresis belongs to less traditional approaches of nucleic acids purification,
… in the application of isotachophoresis for the nucleic acids concentration but also a brief …

Simultaneous purification and fractionation of nucleic acids and proteins from complex samples using bidirectional isotachophoresis

Y Qu, LA Marshall, JG Santiago - Analytical chemistry, 2014 - ACS Publications
… We report on our efforts to create an on-chip system to simultaneously purify and fractionate
nucleic acids and proteins from complex samples using isotachophoresis (ITP). We have …

Nucleic acid sample preparation from whole blood in a paper microfluidic device using isotachophoresis

BP Sullivan, AT Bender, DN Ngyuen, JY Zhang… - … of Chromatography B, 2021 - Elsevier
… based device for purification of nucleic acids from whole blood using isotachophoresis (ITP) …
We show successful extraction and purification of target nucleic acids from large volumes (…

NAIL: Nucleic Acid detection using Isotachophoresis and Loop-mediated isothermal amplification

MD Borysiak, KW Kimura, JD Posner - Lab on a Chip, 2015 - pubs.rsc.org
Nucleic Acid Isotachophoresis LAMP (NAIL) diagnostic device. NAIL uses isotachophoresis
(ITP) and loop-mediated isothermal amplification (LAMP) to extract and amplify nucleic acids

Tube gel isotachophoresis: a method for quantitative isolation of nucleic acids from diluted solutions

VN Kondratova, IV Botezatu, VP Shelepov… - Analytical …, 2011 - Elsevier
… of quantitative isolation of nucleic acid. In our experiments with isotachophoresis on porous
… In the present paper, we report a further development of the tube gel isotachophoresis

Rapid hybridization of nucleic acids using isotachophoresis

M Bercovici, CM Han, JC Liao… - Proceedings of the …, 2012 - National Acad Sciences
… We use isotachophoresis (ITP) to control and increase the rate of nucleic acid hybridization
nucleic acids, and may be applicable to a wide range of reactions involving ionic reactants. …

Bacterial RNA extraction and purification from whole human blood using isotachophoresis

A Rogacs, Y Qu, JG Santiago - Analytical chemistry, 2012 - ACS Publications
extraction and isotachophoresis-based purification of 16S … that the extraction can be
automated using isotachophoresis in … ), and produces a purified total nucleic acid sample that is …