Due to the growing demand for energy in the present-day world, it is obligatory to look for alternative sources of renewable energy. The derivation of power from microbial fuel cells (MFCs) has developed at the vanguard of the alternative source of renewable energy through the concomitant treatment of wastewater. Hence, the process development of MFC is obligatory for creating a sustainable source of renewable energy through the treatment of wastewater. To that end, an attempt was taken in the present study for sustainable power generation from single chamber microbial fuel cell (SCMFC) using Pseudomonas aeruginosa-MTCC-7814. The experiments were carried out in a batch process for 15 days with real dairy wastewater (RDW) having initial chemical oxygen demand (COD) of 8000 mg/L. The open-circuit voltage (OCV) found after 72 h of batch operation was 658 mV, which was maximum within the batch operation. The columbic efficiency (CE) of the batch process was found to be 46.59%. The maximum specific growth rate (μ_max) of Pseudomonas aeruginosa-MTCC-7814 was found to be 0.432 day⁻¹ during batch operation. However, saturation constant (Ks) and inhibition coefficient (K_i) were calculated as 608.74 mg/L, and 6582 mg/L, respectively. The maximum current density (I_max) and saturation constant (K_c) predicted from batch kinetics study were 132 mA/m² and 321 mg/L, respectively, which has resemblance with the data obtained from experiments. The maximum current density and power density from experiments were found to be 161 mA/m² and 34.82 mW/m², respectively. Results showed that a higher power density and current density values were obtained from the present study as compared to the earlier reports that utilized wastewater as the substrate for the MFC. Thus, the study suggests that Pseudomonas aeruginosa, (MTCC-7814) can be used as a promising biocatalyst in MFC for sustainable power generation through the utilization of wastewater treatment.