The state of Gujarat is well known for its contrasting eco-regions such as moist deciduous forests and deserts. The documentation of Lignicolous fungal diversity of Gujarat state was started in 19th century by different scientist. Detailed fungal diversity study was started in 2003 from different parts of Gujarat to identify the lignicolous fungi that destroy wood logs in different forests. During these studies we noticed a great lacuna in fungal diversity of lignicolous fungi occurring in Gujarat. Here we provide, for the first time, lignicolous fungal diversity from timbers of Gujarat state. The survey revealed that the economically important woods present in Gujarat were 14 and locally available common woods were seven. A total of 69 species Lignicolous macro fungi belong to 15 families and 30 general. These wood deteriorating fungi were belonging to the families Xylariaceae, Agaricaceae Coprinaceae, Pleurotaceae Auricularreae, Bondarzewiaceae Ganodermataceae, Shizophyllaceae, Stecherinaceae, Hymenochaetaceae, Lachnocladiaceae, Schizoporaceae, Fomitopsidaceae, Polyporaceae Stereaceae. All the lignicolous fungi were new to Gujarat, India. For the first time Agaricus bisporus (Lange) Sing. Agaricus silvaticus Schaeff. Coprinus plicatilis (Fr.) Fr. Pleurotus pulmonarius (Fries) Quélet, belonging to Agaricales were reported from Gujarat, India. The commonly observed lignicolous fungi on woods belongs to Basidiomycota and Ascomycota were Schizophyllum commune, Flavodon flavus, Ganoderma lucidum, Daldinia concentric, Xylaria polymorpha.