Core Ideas We assessed long‐term manure rates and application times on soil nutrients and
enzymes at two sites. Increasing manure rate enhanced soil fertility but hydrolase enzymes
showed a quadratic trend. Manure application prior to period of heavy rainfall (spring and
fall) was associated with phosphorus losses. Peroxidase and phenol oxidase activities
declined with increasing manure application rates. Oxidative enzyme activities were good
indices of long‐term soil organic carbon storage. We investigated how soil nutrients (soil …