Lysine level in solvent fractions of pearl millet

CC Nwasike, ET Mertz, RC Pickett… - Journal of Agricultural …, 1979 - ACS Publications
CC Nwasike, ET Mertz, RC Pickett, DV Glover, BAK Chibber, SW Van Scoyoc
Journal of Agricultural and Food Chemistry, 1979ACS Publications
MATERIALS AND METHODS The inbred line of pearl millet selected for analysis was one of
168 lines grown at the Purdue Agronomy Farm in the summer of 1975. It is designated as
variety 52731 in the world collection. The whole seeds were finely ground and protein (Nx
6.25) was determined by the microKjeldahl procedure. Fractionation of the seed proteins
was carried out according to the method of Landry and Mour-eaux (1970) as described
indetail earlier (Misra et al., 1975). The individual fractions were obtained by extract-ing …
MATERIALS AND METHODS The inbred line of pearl millet selected for analysis was one of 168 lines grown at the Purdue Agronomy Farm in the summer of 1975. It is designated as variety 52731 in the world collection. The whole seeds were finely ground and protein (Nx 6.25) was determined by the microKjeldahl procedure. Fractionation of the seed proteins was carried out according to the method of Landry and Mour-eaux (1970) as described indetail earlier (Misra et al., 1975). The individual fractions were obtained by extract-ing thefinely ground millet successively with 0.5 M NaCl (fraction I), 70% isopropyl alcohol (fraction II), 70% iso-propyl alcohol containing 2-mercaptoethanol (fraction III), borate buffer (pH 10) containing 2-mercaptoethanol (frac-tion IV), and borate buffer (pH 10) containing 2-mercaptoethanol and sodium dodecyl sulfate (fraction V). Amino acid analysis was conducted according to Spackman et al.(1958).
ACS Publications
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