Methods: One hundred and fifty clinical isolates of NFGNB, confirmed by molecular methods
such as Acinetobacter baumannii [oxa-51 polymerase chain reaction (PCR)], Burkholderia
cepacia complex (expanded multilocus sequence typing) and Stenotrophomonas
maltophilia (species-specific PCR), were taken. Isolated colonies from fresh cultures of all
150 isolates were smeared onto ground steel plate, with and without formic acid extraction
step. The identification was carried out using MALDI-TOF MS Biotyper database. Results: A …

Background & objectives: Matrix-assisted laser desorption ionization-time-of-flight mass
spectrometry (MALDI-TOF MS) has been instrumental in revolutionizing microbiological
identification, especially in high-throughput laboratories. It has enabled the identification of
organisms like non-fermenting Gram-negative bacilli (NFGNB), which has been a
challenging task using conventional methods alone. In this study an attempt was made to
validate MALDI-TOF MS for the identification of clinical isolates of each of the three most …