Mass photometry of membrane proteins

A Olerinyova, A Sonn-Segev, J Gault, C Eichmann… - Chem, 2021 - cell.com
… show that mass photometry can characterize the heterogeneity of membrane proteins and
the … It can also distinguish different functional states of membrane proteins. Our approach thus …

Mass photometry enables label-free tracking and mass measurement of single proteins on lipid bilayers

EDB Foley, MS Kushwah, G Young, P Kukura - Nature Methods, 2021 - nature.com
… These capabilities, together with assay-based advances for studying integral membrane
proteins, will enable the elucidation of biomolecular mechanisms in and on lipid bilayers. …

[HTML][HTML] Biophysical screening pipeline for cryo-EM grid preparation of membrane proteins

S Niebling, K Veith, B Vollmer, J Lizarrondo… - Frontiers in Molecular …, 2022 - frontiersin.org
… After confirming the mass distribution of the sample by mass photometry, our quality
control pipeline (Figure 1) foresees a visual inspection of the sample by negative-stain EM, a …

Characterization of membrane protein interactions by peptidisc-mediated mass photometry

JW Young, E Pfitzner, R van Wee, C Kirschbaum… - Iscience, 2024 - cell.com
membrane protein receptors and their soluble protein binding … membrane protein receptor
and a bactericidal antibody. Our results highlight the utility of peptidiscs for membrane protein

[HTML][HTML] Streamlining Membrane Protein Research with Mass Photometry

C Browne, P Kitchen - lovewomans.com
… of the single membrane protein in solution allows researchers to … identify and compare
membrane protein states under different … involving membrane proteins using mass photometry. …

Molecular assembly and interactions of membrane proteins and viral glycoproteins using mass photometry

A Olerinyova - 2022 - ora.ox.ac.uk
… Integral membrane proteins (IMPs) and their interactions form the basis … mass photometry
as a novel technique to investigate IMPs and their interactions in a variety of membrane

Understanding dynamin polymerization by single-molecule counting and particle-tracking using mass photometry

MS Kushwah, EDB Foley, R Asor, P Kukura - Biophysical Journal, 2022 - cell.com
… Therefore, its involvement together with the concerted action of calcium spikes should not
categorically be excluded, eg in calmodulin interaction with signaling membrane proteins. …

Biophysical characterization of membrane proteins

S Niebling, O Burastero, M García-Alai - Advanced Methods in Structural …, 2023 - Springer
… or aggregational state of a membrane protein in solution [12, … sample is further characterized
by mass photometry (MP). MP … is fast, the membrane protein mass remains adequate during …

Quantifying the heterogeneity of macromolecular machines by mass photometry

A Sonn-Segev, K Belacic, T Bodrug, G Young… - Nature …, 2020 - nature.com
… Respiratory complex I: Respiratory complex I was overproduced in strain BW25113 Δnuo/pBADnuo
nuoF his and solubilization of membrane proteins occurred by adding MNG-3 …

[HTML][HTML] Characterising biomolecular interactions and dynamics with mass photometry

R Asor, P Kukura - Current Opinion in Chemical Biology, 2022 - Elsevier
… , interactions and associated dynamics by mass photometry (MP), the label-free detection and
mass measurement of individual biomolecules in … Mass photometry of membrane proteins