… Derivation and propagation of human embryonic stem (hES) cells from the inner cell mass
… a medium that contained fetal calf serum (FCS), and fetal mouse fibroblasts as feeder cells (…

… Human embryonic stem cell (hESC) … medium components facilitating non-differentiated
growth would be crucial. Conditioned medium most likely contains variable amounts of growth …

… MEF feeder cells may be used to prepare conditioned medium (CM) to support a feeder-free
culture 8 , or HESCs can be grown directly on feeder cells. To confirm that HESC cultures …

… A colony of human embryonic stem cells growing from the inner cell mass of a blastocyst (line
HS351) 12 days after derivation, immediately before the first mechanical splitting. …

… until day 3 in G1 medium, until day 6 in G2.3 medium and then culture of recovered day 6
blastocysts in medium conditioned by buffalo rat liver cells (BRL-medium). Using this protocol, …

… that allowed cell survival through three passages, cells were … -activated cell sorting for
multiple human embryonic stem cell … medium plus 4 ng/ml FGF2; UM 4, unconditioned medium …

… in a serum-free medium. The efficacy in establishing human embryonic stem cell lines
improved in a serum-free medium compared with that in serum-containing media. Four HES …

… of cells like human embryonic stem cells and colonies growing on fibroblast-like cells that
differentiated from the human embryonic stem cell … of serum in the media and later adapted to …

… the human embryonic stem cell (hESC) colonies cultured with eight different xeno-free media.
… -free test media were not able to maintain the undifferentiated growth and the pluripotency …

… The samples were gently washed three times with embryonic stem cell (ESC) medium
before being incubated with the secondary antibodies (Sigma) conjugated to fluorescein …