This review is focused on metabolic mapping in biological tissue with quantitative
bioluminescence and single photon imaging. Metabolites, such as ATP, glucose and lactate,
can be imaged quantitatively and within microscopic dimensions in cryosections from shock
frozen biological specimens using enzyme reactions and light emission by luciferases. The
technique has been applied in numerous targets and models of experimental biomedical
research, such as multicellular spheroids, various organs of laboratory animals in a …