[HTML][HTML] Fusion of FokI and catalytically inactive prokaryotic Argonautes enables site-specific programmable DNA cleavage

Q Wang, GS Rao, T Marsic, R Aman… - Journal of Biological …, 2024 - Elsevier
… Prokaryotic Argonaute (pAgo) proteins have received much attention as biotechnological
tools due to their ability to recognize specific target sequences without a protospacer adjacent …

An Artificial Intelligence-Assisted Argonaute-Mediated Fluorescence Biosensor for the Detection of Viable Salmonella in Food Without DNA Extraction and …

J Zhao, M Han, A Ma, F Jiang, R Chen, Y Dong… - papers.ssrn.com
Accurate detection of viable pathogens is crucial for preventing outbreaks of foodborne
diseases. In this study, we integrated artificial intelligence and single microsphere to develop a …

Catalytic properties and biological function of a PIWI-RE nuclease from Pseudomonas stutzeri

F Huang, X Xu, H Dong, N Li, B Zhong, H Lu… - Bioresources and …, 2022 - Springer
Proteins of the Argonaute (Ago) family with a conserved P-element-induced wimpy testis (…
The conserved oligonucleotide-guided DNA cleavage activity suggests that the N-terminal …

Chimeric CRISPR guides enhance Cas9 target specificity

N Jakimo, P Chatterjee, JM Jacobson - bioRxiv, 2017 - biorxiv.org
… of natural oligonucleotide-guided nucleases associated with … , we later pursued methods
that could bias genomic double-… - which may include DNA-guided editing with Argonaute 42, …

Enzymatic methods for mutation detection in cancer samples and liquid biopsies

F Darbeheshti, GM Makrigiorgos - International Journal of Molecular …, 2023 - mdpi.com
methods based on oligonucleotide-guided enzymes pose robust and versatile platforms in
which some drawbacks of traditional techniquesArgonaute enzyme (NAVIGATER) method, …

Optical Control of Base Editing and Transcription through Light‐Activated Guide RNA

W Zhou, W Brown, A Bardhan, M Tsang… - …, 2021 - Wiley Online Library
… We developed a method to control gene activation and base editing in mammalian cells …
to other oligonucleotide-guided machinery beyond CRISPR/Cas9 and Argonaute/siRNA 18a …

Synthetic circular RNA for protein expression

RA Wesselhoeft - 2019 - dspace.mit.edu
… mRNA transcripts and induce Argonaute-mediated transcript … circularization: chemical
methods using cyanogen bromide … purification, and oligonucleotide-guided RNase H digestion. …

Genome editing in food and feed production–implications for risk assessment. Scientific Opinion of the Scientific Steering Committee of the Norwegian Scientific …

JE Bodin, T Thorstensen, MK Alsheikh, D Basic… - 2021 - imr.brage.unit.no
… how new genome-editing techniques should be regulated … through the use of the new
genome-editing techniques. Therefore, this report describes the use of genome-editing techniques

Structural recognition of the mRNA 3′ UTR by PUF-8 restricts the lifespan of C. elegans

Z Xu, J Zhao, M Hong, C Zeng, S Guang… - Nucleic acids …, 2021 - academic.oup.com
… were determined by molecular replacement using the crystal structure of PUF-8/PBE-5U
as the initial model and modified and refined using the same method used for the PUF-8/PBE-…

Genome editing in food and feed production–implications for risk assessment. Opinion of the Steering Committee of the Norwegian Scientific Committee for Food and …

JE Bodin, T Thorstensen, M Alsheikh, D Basic… - 2021 - oda.oslomet.no
… how new genome-editing techniques should be regulated … through the use of the new
genome-editing techniques. Therefore, this report describes the use of genome-editing techniques