[HTML][HTML] MicroScale Thermophoresis: Interaction analysis and beyond
M Jerabek-Willemsen, T André, R Wanner… - Journal of Molecular …, 2014 - Elsevier
MicroScale Thermophoresis (MST) is a powerful technique to quantify biomolecular
interactions. It is based on thermophoresis, the directed movement of molecules in a
temperature gradient, which strongly depends on a variety of molecular properties such as
size, charge, hydration shell or conformation. Thus, this technique is highly sensitive to
virtually any change in molecular properties, allowing for a precise quantification of
molecular events independent of the size or nature of the investigated specimen. During a …
interactions. It is based on thermophoresis, the directed movement of molecules in a
temperature gradient, which strongly depends on a variety of molecular properties such as
size, charge, hydration shell or conformation. Thus, this technique is highly sensitive to
virtually any change in molecular properties, allowing for a precise quantification of
molecular events independent of the size or nature of the investigated specimen. During a …
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