Microtubule growth activates Rac1 to promote lamellipodial protrusion in fibroblasts
CM Waterman-Storer, RA Worthylake, BP Liu… - Nature cell …, 1999 - nature.com
Nature cell biology, 1999•nature.com
Microtubules are involved in actin-based protrusion at the leading-edge lamellipodia of
migrating fibroblasts. Here we show that the growth of microtubules induced in fibroblasts by
removal of the microtubule destabilizer nocodazole activates Rac1 GTPase, leading to the
polymerization of actin in lamellipodial protrusions. Lamellipodial protrusions are also
activated by the rapid growth of a disorganized array of very short microtubules induced by
the microtubule-stabilizing drug taxol. Thus, neither microtubule shortening nor long-range …
migrating fibroblasts. Here we show that the growth of microtubules induced in fibroblasts by
removal of the microtubule destabilizer nocodazole activates Rac1 GTPase, leading to the
polymerization of actin in lamellipodial protrusions. Lamellipodial protrusions are also
activated by the rapid growth of a disorganized array of very short microtubules induced by
the microtubule-stabilizing drug taxol. Thus, neither microtubule shortening nor long-range …
Abstract
Microtubules are involved in actin-based protrusion at the leading-edge lamellipodia of migrating fibroblasts. Here we show that the growth of microtubules induced in fibroblasts by removal of the microtubule destabilizer nocodazole activates Rac1 GTPase, leading to the polymerization of actin in lamellipodial protrusions. Lamellipodial protrusions are also activated by the rapid growth of a disorganized array of very short microtubules induced by the microtubule-stabilizing drug taxol. Thus, neither microtubule shortening nor long-range microtubule-based intracellular transport is required for activating protrusion. We suggest that the growth phase of microtubule dynamic instability at leading-edge lamellipodia locally activates Rac1 to drive actin polymerization and lamellipodial protrusion required for cell migration.
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