… working in the area of biofilms, we present a guideline for the spectrophotometric and
fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 …

… a completely dry sample with minimal disturbance of the biofilm or substrate. After drying, the
… Plate reader or UV/VIS spectrophotometer: Gram Stain Indirect measure of biofilm growth. …

… methods. First, we addressed the issue of selective reporting through the application of …
minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm …

… of biofilms in 96-well-plates and determined minimal biofilm … Some methods involve staining
(eg, with Crystal Violet (CI … ) to determine cell viability in biofilms via spectrophotometric …

… , especially for spectrophotometric and fluorometric type assays. This combined with more
detailed reporting based on minimum information guidelines could add more value to results …

… area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment
of biofilm for- … This guideline has been divided into 5 main sections, each presenting a …

… methods, to quantify total formed biofilms [7–9]. The most common system used to assess
biofilm formation is a spectrophotometer, … At least three biological replicates were included for …

… was grown individually in Minimal Medium (basal medium … then colonies were transferred
onto Minimal Medium agar plates … % ethanol and measured spectrophotometrically. The data …

… Materials and methods The microplate experiments conformed to the minimum information
guideline for spectrophotometric and fluorometric methods to assess biofilm formation in …

… minimum reporting guidelines may benefit the literature. … Such minimum reporting information
standards for experiments … report spectrophotometric and fluorometric methods to assess …