Mn2+ Quenching Assay for Store-Operated Calcium Entry

Z Pan, S Choi, Y Luo - The CRAC Channel: Methods and Protocols, 2018 - Springer
Z Pan, S Choi, Y Luo
The CRAC Channel: Methods and Protocols, 2018Springer
Abstract Store-operated Ca 2+ entry (SOCE) pathway plays important roles in many cellular
processes, which is largely studied by using fluorescent Ca 2+ indicator, Fura-2.
Extracellular Mn 2+ is able to cross the plasma membrane through SOCE and quenches the
fluorescence signals from Fura-2. Thus, the fluorescence quenching rate by Mn 2+
composes a convenient assay to monitor the extent of SOCE. This chapter describes an
experimental method of Mn 2+ quenching assay for both cultured esophageal epithelial and …
Abstract
Store-operated Ca2+ entry (SOCE) pathway plays important roles in many cellular processes, which is largely studied by using fluorescent Ca2+ indicator, Fura-2. Extracellular Mn2+ is able to cross the plasma membrane through SOCE and quenches the fluorescence signals from Fura-2. Thus, the fluorescence quenching rate by Mn2+ composes a convenient assay to monitor the extent of SOCE. This chapter describes an experimental method of Mn2+ quenching assay for both cultured esophageal epithelial and skeletal muscle cells. It also explains how to perform a quantitative analysis of graded SOCE.
Springer
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