Wireless communication has been growing exponentially over the last decade, where multimedia application, satellite and radar communication have developing with it, as well as demand for multitask features in same device has drawn attention to the consumers. Integration of Microstrip Patch Antenna in wireless equipments helps to fulfill requirement due to its miniature dimension, robustness and inexpensive attributes. In this paper, a modified slots loaded with E shaped patch antenna with dielectric substrate layer on the top has been illustrated. The proposed antenna has the capability to operate in eight different multiple frequencies having single radiator. The antenna has been design on the top of the dielectric substrate of permittivity 4.3 and thickness of 1.6 mm. The proposed antenna resonates at 5.54 GHz, 6.51 GHz, 7.75 GHz, 8.15 GHz, 10.49 GHz, 11.98 GHz, 12.96 GHz and 16.11 GHz with impedance bandwidth of 44.51 MHz, 33.28 MHz, 330 MHz, 102 MHz, 1.101 GHz, 483 MHz and 4.532 respectively that covers C, X and Ku band. The design scheme and simulation results of proposed antenna are presented.