Molecular evidence for association ofTomato leaf curl New Delhi virus with leaf curl disease of papaya (Carica papaya L.) in India

SK Raj, SK Snehi, MS Khan, R Singh… - Australasian Plant Disease …, 2008 - Springer
Australasian Plant Disease Notes, 2008Springer
… tabaci from naturally infected papaya to healthy seedlings of papaya, tobacco, tomato
and chilli and all the inoculated test species displayed typical leaf curl symptoms indicating
the association of a whitefly transmissible infectious agent, possibly a begomovirus, with the
leaf curl disease of papaya. For … To confirm the authenticity of the ~770 bp amplicons, the
gel was blotted onto nylon membrane and was allowed to hybridise with a probe of a CP
clone of Tomato leaf curl New Delhi virus (DQ431846) which showed strong signals of …
Abstract
Association ofTomato leaf curl New Delhi virus with leaf curl disease of papaya (Carica papaya L.) was detected by polymerase chain reaction using begomovirus-specific primers and confirmed by highest sequence similarities and close phylogenetic relationships.
Springer
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