Sheep gastrointestinal tract (GIT) plays a vital role in storage and microbial digestion of ingested feedstuffs which is indispensible for fulfilling the energy requirement and maintenance of body functionality. The histo-morphometry and biometry of GIT of indigenous sheep were studied. Samples were collected from the GIT ie rumen, reticulum, cecum and colon of five indigenous sheep in Bangladesh. The samples were collected immediately after slaughtering. The samples were stained with Hematoxylin and Eosin. The tissues were examined under light microscope and photographs were taken by digital camera. The biometric measurements of different parts of the GIT were performed using calibrated stage micrometer. Morphologically, the GIT wall was comprised of mucosa, submucosa, muscularis and serosa. The mucosa of rumen and reticulum revealed numerous papillae. Each papilla lined by stratified squamous epithelium inrumen and reticulum. The average length and width of papillae were more in rumen than in reticulum. The lamina muscularis was absent in rumen. The submucosa comprised of irregular connective tissues. The mucosa of cecum and colon throwned into numerous leaf shaped folds lined by simple columnar epithelia with large number of goblet cells. The lamina propria filled with crypts of lieberkuhn in cecum and colon. The diameter of crypts of lieberkuhn was more in cecum than in colon which was statistically non significant. Tunica muscularis of the GIT composed of two layers of smooth muscle bundles in rumen, reticulum, cecum and colon. The average length of tunica muscularis was the highest in rumen and the lowest in cecum. The serosa consisted of loose connective tissues and mesothelium. Data obtained frombiometric study of GIT were recorded in sheep. In conclusion, the morphometric and biometric study of GIT provide necessary information for the practicing veterinary gastroenterologist and researchers.