Yarrowia lipolytica is one of the most extensively studied nonconventional yeasts.
Unfortunately, few methods for gene disruption have been reported for this yeast, and all of
them are time-consuming and laborious. The functional analysis of unknown genes requires
powerful disruption methods. Here, we describe such a new method for rapid gene
disruption in Y. lipolytica. This knockout system combines SEP method and the Cre-lox
recombination system, facilitating efficient marker rescue. Versatility was increased by using …