Current sequencing platforms are characterized by an increased throughput of data, short
reads, and low costs. These platforms have changed the way genome studies are
conducted because the information from a full eukaryote genome can be sequenced in a
single run with several times coverage. Many areas in genomics have been adapted to work
with the short reads produced by these new platforms. For example, in the de novo and
reference assembly of genomes, new algorithms have been developed to handle short …

The emergence of next-generation sequencing (NGS) technologies, starting in 2005, led to
major advances in genomics and improvements in contrast to the Sanger method, such as a
massively parallel sequencing capacity, high-throughput sequencing, and cost reductions
(Liu et al., 2012). Among the new sequencing technologies that have been released over
the last 10 years, the most commonly used are the 454 GS FLX (Roche), HiSeq (Illumina),
SOLiD (Sequencing by Oligonucleotide Ligation and Detection; Thermo Fisher Scientific) …