Background Nitric oxide (NO·) derived from donor drugs has been shown to be an effective
chemosensitizer in vitro. We investigated the combination of inducible nitric oxide synthase
(iNOS) gene transfer, driven by a strong constitutive promoter (cytomegalovirus; CMV) with
the DNA cross‐linking agent cisplatin in mouse and human tumour cell lines. Methods Proof
of principal experiments were performed in the radiation‐induced fibrosarcoma‐1 (RIF‐1)
murine cell line. Cells were transfected with constitutively expressed CMV/iNOS plasmid …

BACKGROUND AIMS: Cell-based gene therapy is an alternative to viral and non-viral gene
therapy. Emerging evidence suggests that mesenchymal stem cells (MSC) are able to
migrate to sites of tissue injury and have immunosuppressive properties that may be useful
in targeted gene therapy for sustained specific tissue engraftment. METHODS: In this study,
we injected intravenously (iv) 1x10 (6) MSC, isolated from green fluorescent protein (GFP)
transgenic rats, into Rif-1 fibrosarcoma-bearing C3H/HeN mice. The MSC had been infected …