Sixty female and 30 male Japanese quails, 6 weeks old, were used to study the relationship between vitamin E (vit E) and selenium (Se) during the laying period. Birds were divided into 30 groups each of 2 females and 1 male. The productive units were divided into 5 groups, each of six birds. The experiment started at 6 and was terminated at 27 weeks of age. The experimental period (6 to 27 weeks) was divided into three phases: from 6 to 12, 13 to 20 and 21 to 27 weeks. Birds in treatment 1 served as the control and were fed a control diet. Birds of the other four treatments received vit E and Se (as sodium selenite) per kg of diet: 25 mg vit E, 1 mg Se; 25 mg vit E, 2 mg Se; 50 mg vit E, 1 mg Se; and 50 mg vit E, 2 mg Se for treatments 2, 3, 4 and 5, respectively. Results indicated that supplementing the laying diet with vit. E and Se improved (P<0.05) egg production (number and weight), feed conversion, fertility, hatchability, total lipids, total protein, globulin and alkaline phosphatase in blood plasma. Addition of vit E and Se did not significantly affect feed intake or plasma albumin. The best improvement in productive and plasma constituents occurred when Se was added at 1 mg/kg diet with vit E at 25 or 50 mg/kg.