On the fidelity of DNA replication. Effect of the next nucleotide on proofreading.
TA Kunkel, RM Schaaper, RA Beckman… - Journal of Biological …, 1981 - ASBMB
The contribution of proofreading to the fidelity by which Escherichia coli DNA polymerase I
copies natural DNA has been analyzed by two independent criteria. With phi X174 am 3
DNA as a template, there is approximately a 25-fold increase in noncomplementary base
substitutions at position 587 when the concentration of the next correct nucleotide, dATP, is
increased. Sequence analysis indicates that the mistakes represent misincorporation of C in
place of T at position 587. This mutagenic response is presumed to result from a decrease in …
copies natural DNA has been analyzed by two independent criteria. With phi X174 am 3
DNA as a template, there is approximately a 25-fold increase in noncomplementary base
substitutions at position 587 when the concentration of the next correct nucleotide, dATP, is
increased. Sequence analysis indicates that the mistakes represent misincorporation of C in
place of T at position 587. This mutagenic response is presumed to result from a decrease in …
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