Tannin acyl hydrolase (TAH), commonly referred as tannase (EC. 3.1. 1.20) is involved in tannins biodegradation, has important roles in various applications, particularly in food and pharmaceutical industries. Twenty six fungal isolates were screened for their tannase production. One isolate (TL1) produced 0.34 U/ml of tannase was found to be the most potent and subjected for further identification to be identified as Aspergillus flavus var. columnaris. A medium optimization was carried out for maximum tannase productivity, 5.18 U/ml, under solid state fermentation (SSF) ie substrate concentration of 0.3 g/ml of wheat bran (WB), pH 3.8, incubation period 4 days at 30 C. The best carbon & nitrogen sources were maltose and NH4Cl respectively. Different heavy spore suspension inocula were tested and 0.5 ml/10 ml was the best one that gave maximum tannase production with concentration of 3% of tannic acid. Different purification steps were carried out with purification folds of 5.8 times from the origin and 4.7% of recovery yield. The purification steps were followed by Amino acid analysis of the purified tannase which indicated that, Phenyle alanin represented the highest one ie 947.6 μg/ml. SDS-PAGE of the purified enzyme revealed that the molecular weight of tannase was about 68 KDa. Optimization of the purified enzyme was achieved at enzyme thermostability 40 C, pH stability 6 when incubated for 80 min with (1.84 mg/ml) of methyle gallate in the presence of 1 ml of tannase produced by Aspergillus flavus var. columnaris.[Roushdy MM, Desouky SE, Esmael ME, Elboudy SS, Elshikh HH. Optimization and Characterization of