Optimization of Fe 3 O 4 nanozyme activity via single amino acid modification mimicking an enzyme active site

K Fan, H Wang, J Xi, Q Liu, X Meng, D Duan… - Chemical …, 2017 - pubs.rsc.org
K Fan, H Wang, J Xi, Q Liu, X Meng, D Duan, L Gao, X Yan
Chemical Communications, 2017pubs.rsc.org
The Fe3O4 nanozyme was the first reported nanoparticle with intrinsic peroxidase-like
activity and has been widely used in biomedicine. To optimize its catalytic activity, we
introduced histidine residues onto the Fe3O4 nanoparticle surface in order to mimic the
enzymatic microenvironment of natural peroxidase enzymes. Our results show that
modification with a single amino acid could more than ten-fold improve the apparent affinity
(KM) of the Fe3O4 nanozyme for the substrate H2O2 and enhanced its catalytic efficiency …
The Fe3O4 nanozyme was the first reported nanoparticle with intrinsic peroxidase-like activity and has been widely used in biomedicine. To optimize its catalytic activity, we introduced histidine residues onto the Fe3O4 nanoparticle surface in order to mimic the enzymatic microenvironment of natural peroxidase enzymes. Our results show that modification with a single amino acid could more than ten-fold improve the apparent affinity (KM) of the Fe3O4 nanozyme for the substrate H2O2 and enhanced its catalytic efficiency (kcat/KM) up to twenty fold. Thus we not only optimized the activity of the Fe3O4 nanozyme, but also provide a new rationale for improving the efficiency of nanomaterial-based catalysts by utilizing strategies observed in nature.
The Royal Society of Chemistry
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