[PDF][PDF] Optimization of factors affecting organogenesis and somatic embryogenesis of Anthurium andreanum lind. Tera

E Beyramizade, P Azadi, M Mii - Propagation of Ornamental Plants, 2008 - researchgate.net
E Beyramizade, P Azadi, M Mii
Propagation of Ornamental Plants, 2008researchgate.net
In vitro propagation of Anthurium has special importance for rapid clonal propagation and
disease elimination. In this research, micropropagation of Anthurium andreanum 'Tera'was
investigated through organogenesis and somatic embryogenesis. For organogenesis, best
result for callus induction was obtained on half strength MS medium containing 0.08 mg l-1
2, 4-D and 1 mg l-1 BA, whereas medium without phytohormones induced the highest
number of shoots from the callus. Plantlets were successfully transferred to pots and grown …
Abstract
In vitro propagation of Anthurium has special importance for rapid clonal propagation and disease elimination.
In this research, micropropagation of Anthurium andreanum ‘Tera’was investigated through organogenesis and somatic embryogenesis. For organogenesis, best result for callus induction was obtained on half strength MS medium containing 0.08 mg l-1 2, 4-D and 1 mg l-1 BA, whereas medium without phytohormones induced the highest number of shoots from the callus. Plantlets were successfully transferred to pots and grown in the greenhouse. For somatic embryogenesis, the highest percentage of somatic embryos was observed on medium containing 3 mg l-1 2, 4-D and 0.33 mg l-1 kinetin. Conversion and maturation of embryos occurred on the same basal medium with 0.4 mg l-1 A. After germination on phytohormone-free medium, plantlets were transferred to in vivo condition and grown to normal plants on a perlite bed.
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