The study was aimed to determine the role of pulsed electric field (PEF) treatment before hydro-distillation of the patchouli oil. Response Surface Methodology (RSM) was employed to optimize PEF treatment (voltages, frequencies) and times of distillation of patchouli oil from dried patchouli crops. The experimental design and analysis the result to obtain the optimal processing parameters was a Box-Behnken Design (BBD). Three variables were examined in this study: voltages (1,000-2,000 volt); frequencies (1,000-2,000 Hz); and distillation time (4-8 hours). The results showed that the voltage greatly affects the volume of patchouli oil obtained and optimum condition of PEF was voltages of 2,000 volts, frequencies of 1,874 Hz, and 8 hours distillation. The patchouli oil obtained is 8.037 ml of 300 g of dry material (±2.7%). The verification of the model shows that 96.6%(7.76±0.15 ml) can adequately for reflecting the expected optimization.