Optogenetic control of kinetochore function

H Zhang, C Aonbangkhen, EV Tarasovetc… - Nature Chemical …, 2017 - nature.com
… two important kinetochore functions: checkpoint signaling and molecular motor activity.
Overall, our findings establish a foundation for opotogenetic manipulation of kinetochores and …

Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces

M Jagrić, P Risteski, J Martinčić, A Milas, IM Tolić - Elife, 2021 - elifesciences.org
… by the forces exerted by kinetochore microtubules and polar ejection … kinetochore fibers
with bridging fibers in chromosome alignment is unknown. Here, we develop an optogenetic

Optogenetic control of PRC1 reveals its role in kinetochore alignment at the metaphase plate

M Jagrić, P Risteski, J Martinčić, A Milas, IM Tolić - bioRxiv, 2019 - biorxiv.org
… by the forces exerted by kinetochore microtubules and polar ejection … kinetochore fibers
with bridging fibers in chromosome alignment is unknown. Here we develop an optogenetic

[PDF][PDF] Optogenetic control of PRC1 reveals that bridging fibers promote chromosome alignment by overlap length-dependent forces

M Jagrić, P Risteski, J Martinčić, A Milas, IM Tolic - bioRxiv, 2019 - scholar.archive.org
… by the forces exerted by kinetochore microtubules and polar ejection … kinetochore fibers
with bridging fibers in chromosome alignment is unknown. Here we develop an optogenetic

Lights, cytoskeleton, action: Optogenetic control of cell dynamics

T Wittmann, A Dema, J van Haren - Current Opinion in Cell Biology, 2020 - Elsevier
… protein functions in living cells. We provide a brief overview of various types of optogenetic
switches. We then discuss how diverse approaches have been used to control cytoskeleton …

[HTML][HTML] Opto-katanin, an optogenetic tool for localized, microtubule disassembly

JCM Meiring, I Grigoriev, W Nijenhuis, LC Kapitein… - Current Biology, 2022 - cell.com
… Since p60-severing activity depends on its recruitment to microtubules, we considered
this enzyme to be a promising candidate for an optogenetic tool. We tested an assortment of …

Optogenetic rescue of a patterning mutant

HE Johnson, NJV Djabrayan, SY Shvartsman… - Current Biology, 2020 - cell.com
… Here, we demonstrate exactly this capability by combining optogenetic control of Ras/extracellular
signal-related kinase (ERK) signaling with the genetic loss of the receptor tyrosine-…

Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells

A Milas, M Jagrić, J Martinčić, IM Tolić - Methods in cell biology, 2018 - Elsevier
… Thus, our approach of optogenetic control of Kif18A localization led to association of …
the optogenetic knocksideways, we can study the dynamics of the cut kinetochore fibers …

Optogenetic control of mitosis with photocaged chemical dimerizers

H Zhang, DM Chenoweth, MA Lampson - Methods in cell biology, 2018 - Elsevier
… We describe an optogenetic platform, based on photosensitive chemical inducers of dimerization,
which provides control over dimerization of … Optogenetic control of kinetochore function

Optogenetic EB1 inactivation shortens metaphase spindles by disrupting cortical force-producing interactions with astral microtubules

A Dema, J van Haren, T Wittmann - Current Biology, 2022 - cell.com
… Different microtubule populations contribute to spindle function: kinetochore microtubules
attach and transmit forces to chromosomes, … Optogenetic control of microtubule dynamics. …