Animals used for this third experiment were also selected among those survivors of the
growth study. To evaluate their capacity to osmoregulate in response to environmental
stresses, shrimp in “C” and “D0” molt stage were subjected to combined salinity/temperature
shock (temperature dropped from 28 C to 17 C for 12 hours, then salinity decreased from
35% to 13% for 24 hours). Haemolymph samples were collected after an eight-hour
starvation period. Haemolymph was sampled with a needle of a 1-ml hypodermic syringe …