The present investigation intended to evaluate the pharmacognostical features and physicochemical analysis of Barleria gibsoni Dalz.(B. gibsoni) leaves. Macroscopic, microscopic and physicochemical studies such as moisture content, ash values, extractive values were carried out as recommended by WHO. Microscopical studies revealed the presence of stomata, trichomes, vascular bundles and calcium oxalate crystals. Physicochemical parameters such as moisture content, extractive values, ash content and fluorescent studies were also determined. This is the first report on the pharmacognostic and physicochemical studies of B. gibsoni and is helpful in the characterization of the crude drug.