It is unclear how cancer cells coordinate glycolysis and biosynthesis to support rapidly
growing tumors. We found that the glycolytic enzyme phosphoglycerate mutase 1 (PGAM1),
commonly upregulated in human cancers due to loss of TP53, contributes to biosynthesis
regulation in part by controlling intracellular levels of its substrate, 3-phosphoglycerate (3-
PG), and product, 2-phosphoglycerate (2-PG). 3-PG binds to and inhibits 6-
phosphogluconate dehydrogenase in the oxidative pentose phosphate pathway (PPP) …

The current understanding of the Warburg effect consists of an increase in aerobic glycolysis
in cancer cells. The connection between glycolysis and PPP/biosynthesis is based upon a
model in which glycolytic intermediates can be diverted into PPP and biosynthesis pathways
as precursors. However, it remains unclear how cancer cells coordinate glycolysis and
biosynthesis to fulfill the request of rapidly growing tumors. We found that glycolytic enzyme
phosphoglycerate mutase 1 (PGAM1), commonly upregulated in human cancers due to loss …