Phylogenetic diversity of bacteria in the leachate of a full-scale recirculating landfill

LN Huang, H Zhou, S Zhu, LH Qu - FEMS Microbiology Ecology, 2004 - academic.oup.com
We analyzed the phylogenetic composition of bacterial community in the effluent leachate of
a full-scale recirculating landfill using a culture-independent molecular approach. 16S rRNA
genes were amplified directly from leachate DNA with universally conserved and Bacteria-
specific rDNA primers and cloned. The clone library was screened by restriction fragment
length polymorphism, and representative rDNA sequences were determined. Many bacterial
sequences displaying relatively low levels of similarity to any other hitherto reported rDNA …

Phylogenetic diversity of bacteria in the leachate of a full-scale recirculating landfill.

HLN Huang LiNan, ZH Zhou Hui, ZSA Zhu ShuAng… - 2004 - cabidigitallibrary.org
We analyzed the phylogenetic composition of bacterial community in the effluent leachate of
a full-scale recirculating landfill using a culture-independent molecular approach. 16S rRNA
genes were amplified directly from leachate DNA with universally conserved and Bacteria-
specific rDNA primers and cloned. The clone library was screened by restriction fragment
length polymorphism, and representative rDNA sequences were determined. Many bacterial
sequences displaying relatively low levels of similarity to any other hitherto reported rDNA …
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