Callus induction and regeneration in vitro of three rice varieties (Oryza sativa L. ssp indica), Bg 94-1, Moraberekon and Bg 300 were examined. Phenotypic variations of regenerated and parent plants were compared and the method of plant regeneration was confirmed by histology. Callusing was induced by culturing the mature embryos of the three rice varieties in a liquid medium of distilled water containing 13.2 mg/L 2, 4-D and 2% sucrose for 7 days. Calli were proliferated for three weeks on solidified MS medium containing NAA and BAP at 1 mg/L each and 2% sucrose. Regeneration of calli was induced by culture solidified MS medium containing 100 mg/L tryptophan and 3% sucrose. The proliferation and regeneration media were solidified using 3 g/L gelrite. Regeneration was further stimulated by callus dehydration using silica gel and culture for 3 days on the latter medium solidified by 1% agarose. Callus induction frequencies of Bg 94-1, Moraberekon and Bg 300 were 61%, 67.5% and 55% respectively. The shoot bud induction frequencies of these varieties were 6.5%, 7.5% and 7.5% respectively. Regeneration frequencies of Bg 94-1 and Moraberekon were 100% and 43% respectively. Bg 300 calli did not regenerate. The comparison of regenerated and parent plants at a phenotypic level on maximum tiller number, maximum height and seed weight, indicated that the regenerated plants of Bg 94-1 were similar to parent plants, while Moraberekon showed variations, compared to parent plants. Histological studies revealed that regeneration occurred via indirect organogenesis from calli initiated from the rice scutellum.