The concept of a thin cell layer (TCL) was initially presented by Tran Thanh Van in two key
papers exactly 40 years ago. At that time, Nicotiana tabacum was the model plant used to
establish three main pathways for de novo organogenesis by developing a flower,
vegetative bud, and root “programme” from pedicel tissue. Over the last 40 years, a wealth of
research in plant tissue culture based on TCLs has emerged, fortifying the importance of this
very simple technique, and highlighting its fundamental importance as a key tool in plant cell …

The concept of a thin cell layer (TCL) was initially presented by Tran Thanh Van in two key
papers exactly 40 years ago. At that time, Nicotiana tabacum was the model plant used to
establish three main pathways for de novo organogenesis by developing a flower,
vegetative bud, and root “programme” from pedicel tissue. Over the last 40 years, a wealth of
research in plant tissue culture based on TCLs has emerged, fortifying the importance of this
very simple technique, and highlighting its fundamental importance as a key tool in plant cell …