Acute encephalitis syndrome (AES) is be defined as the acute-onset of fever with change in mental status (including symptoms such as confusion, disorientation, coma, or inability to talk) and often with new onset of seizures (excluding simple febrile convulsion) in a person of any age at any time of the year (Solomon et al., 2008). It is a major health problem in Asia. AES includes illness caused by many infectious as well as non infectious causes and most are considered as viral encephalitis (Jmor et al., 2008). JE has been considered as leading cause of AES in India (Gendelman and Persidsky, 2005; Das, 2005). The main etiological agent is Japanese encephalitis virus (JEV), a positive sense single stranded zoonotic flavivirus transmitted by Culex spp. mosquitoes (Geevarghese et al., 2004) between wild/domestic birds and pigs; where birds act as reservoir host and pig act as amplifying host (Reuben and Gajanana, 1997). Man is the accidental host and dead end for the transmission of the disease (Diagana et al., 2007; Scherer