Potentiometric DNA sensor based on electropolymerized phenothiazines for protein detection
GA Evtugyn, AV Porfireva, T Hianik… - … Journal Devoted to …, 2008 - Wiley Online Library
Novel method of potentiometric detection of DNA‐protein interactions has been proposed.
For this purpose, polymeric phenothiazine dyes, methylene blue (MB) and methylene green
(MG), were electrochemically deposited onto the glassy carbon electrode and covered with
double stranded DNA (dsDNA) as a target for antibodies (DNA‐sensor) or DNA aptamer
specific to human α‐thrombin (aptasensor). The biosensors were consecutively incubated at
pH 7.5 and 3.0 and the difference in potentials, ΔE, was used as a measure of protein …
For this purpose, polymeric phenothiazine dyes, methylene blue (MB) and methylene green
(MG), were electrochemically deposited onto the glassy carbon electrode and covered with
double stranded DNA (dsDNA) as a target for antibodies (DNA‐sensor) or DNA aptamer
specific to human α‐thrombin (aptasensor). The biosensors were consecutively incubated at
pH 7.5 and 3.0 and the difference in potentials, ΔE, was used as a measure of protein …
Potentiometric DNA sensor based on electropolymerized phenothiazines for protein detection
A Porfireva, M Cheburova, H Budnikov, T Hianik… - dspace.kpfu.ru
Novel method of potentiometric detection of DNA-protein interactions has been proposed.
For this purpose, polymeric phenothiazine dyes, methylene blue (MB) and methylene green
(MG), were electrochemically deposited onto the glassy carbon electrode and covered with
double stranded DNA (dsDNA) as a target for antibodies (DNA-sensor) or DNA aptamer
specific to human α-thrombin (aptasensor). The biosensors were consecutively incubated at
pH 7.5 and 3.0 and the difference in potentials, ΔE, was used as a measure of protein …
For this purpose, polymeric phenothiazine dyes, methylene blue (MB) and methylene green
(MG), were electrochemically deposited onto the glassy carbon electrode and covered with
double stranded DNA (dsDNA) as a target for antibodies (DNA-sensor) or DNA aptamer
specific to human α-thrombin (aptasensor). The biosensors were consecutively incubated at
pH 7.5 and 3.0 and the difference in potentials, ΔE, was used as a measure of protein …
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