Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genome
… of 27 previously published SARS-CoV-2 diagnostic assays including those recommended
by … show mismatches in seven assays, highlighting the need for keeping the assay current …
by … show mismatches in seven assays, highlighting the need for keeping the assay current …
Thermodynamic evaluation of the impact of DNA mismatches in PCR-type SARS-CoV-2 primers and probes
P Miranda, G Weber - Molecular and cellular probes, 2021 - Elsevier
… SARS-CoV-2 and to 21665 SARS-CoV-2 genomes as well as 323 genomes of other viruses
of the coronavirus … that occur in the SARS-CoV-2 genome [31,32] imply that the presence of …
of the coronavirus … that occur in the SARS-CoV-2 genome [31,32] imply that the presence of …
Mutations in animal SARS-CoV-2 induce mismatches with the diagnostic PCR assays
… acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was first … Despite the presence
of an RNA proofreading … A total of 793 SARS-CoV-2 genomes isolated from animals were …
of an RNA proofreading … A total of 793 SARS-CoV-2 genomes isolated from animals were …
Discrepancy between PCR based SARS-CoV-2 tests suggests the need to re-evaluate diagnostic assays
MZ Mushtaq, S Shakoor, A Kanji, N Shaheen… - BMC research …, 2021 - Springer
… indicate the presence of new SARS-CoV-2 variant strains. … ], as well as diagnostic primer/probe
mismatches with infecting … have genome sequences available of the SARS-CoV-2 strains …
mismatches with infecting … have genome sequences available of the SARS-CoV-2 strains …
Clinical and laboratory diagnosis of SARS-CoV-2, the virus causing COVID-19
SJR da Silva, CTA Silva, KM Guarines… - ACS infectious …, 2020 - ACS Publications
… some viruses and inhibit PCR testing. When collection with a … the detection of the SARS-CoV-2
genome in patient samples. … world identified the presence of mutations/mismatches in the …
genome in patient samples. … world identified the presence of mutations/mismatches in the …
Nucleic acid testing of SARS-CoV-2
HM Yoo, IH Kim, S Kim - International Journal of Molecular Sciences, 2021 - mdpi.com
… genomic sequence similarity of RATG13 to SARS-CoV-2 … , or turbidity indicate the presence
of SARS-CoV-2 RNA or DNA [… to the sequence mismatch hinders the genome sequencing of …
of SARS-CoV-2 RNA or DNA [… to the sequence mismatch hinders the genome sequencing of …
Thermodynamic assessment of DNA mismatches in PCR and LAMP primers for SARS-CoV-2 detection and their effectiveness to variants
PM de Moura - 2024 - repositorio.ufmg.br
… if the presence of DNA mismatches in both RT-PCR and RT-… Primer-target mismatches can
impact the PCR assay in a few … a considerable set of SARS-CoV-2 genomes collected in the …
impact the PCR assay in a few … a considerable set of SARS-CoV-2 genomes collected in the …
[HTML][HTML] Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2
D Li, J Zhang, J Li - Theranostics, 2020 - ncbi.nlm.nih.gov
… were designed from the SARS-CoV-2 genome sequence and … for the presence of all known
and potentially unknown … in the SARS-CoV-2 genomes, there may be mismatches in primers …
and potentially unknown … in the SARS-CoV-2 genomes, there may be mismatches in primers …
New RT-PCR assay for the detection of current and future SARS-CoV-2 variants
A Marchini, M Petrillo, A Parrish, G Buttinger, S Tavazzi… - Viruses, 2023 - mdpi.com
… for the routine monitoring of SARS-CoV-2’s presence in a … PCR performance from the other
mismatches found for other … in sequencing the viral genome to closely monitor SARS-CoV-2’s …
mismatches found for other … in sequencing the viral genome to closely monitor SARS-CoV-2’s …
rRT-PCR for SARS-CoV-2: Analytical considerations
… on the presence of haplotypes and mutations in virus genome … , which can explain the reason
of the mismatch between the … in the SARS-CoV-2 genome that might impact on PCR assay …
of the mismatch between the … in the SARS-CoV-2 genome that might impact on PCR assay …