Blood samples from 1,000 subjects, 2-80 years old, were tested to explore the prevalence of alpha-thalassemia trait, high persistent HbF (HPFH), sickle cell trait and beta-thalassemia minor in northern Jordan. Hematological parameters and hemoglobin electrophoresis were carried out on all samples. Results showed 10 (1%) subjects were sickle cell trait, 35 (3.5%) were heterozygous beta thalassemia, 31 (3.1%) alpha thalassemia trait and 10 (1%) high persistent HbF. The prevalence rates were different from those reported in neighbouring countries and their significance is discussed. No other abnormal hemoglobin types were observed.