he prevalence of porcine circovirus 2 (PCV-2) infection in the pig population in Slovakia was investigated. Sera from pigs suspected for post-weaning multisystemic wasting syndrome (PMWS) as well as clinically healthy pigs were tested for viral DNA and specific IgM and IgG antibodies. Pigs (n= 198) were categorized to weaning, grower and fattening ones and sows. he results showed that PCV-2 antibodies were present in 53.4% of PMWS-suspects, in 50.0% of healthy pigs and in 69.0% of sows. In PMWS-suspect grower pigs, 40.7% were positive for IgM+ IgG antibodies and 22.2% for viral DNA. In PMWS-suspect fattening pigs, 50.0% were positive for IgM+ IgG antibodies and 25.0% for viral DNA. In healthy fattening pigs, almost 90.0% were positive for IgG antibodies and 38.5% for viral DNA. he highest proportion of PMWS-suspects was in grower pigs and specific antibodies were increasing with the age of pigs. A combination of positivities for IgG+ IgM antibodies and viral DNA was a highly significant marker of PMWS. Viral DNA was detected in seropositive as well as seronegative PMWS-suspects. Overall, in all categories of pigs tested, specific antibodies and viral DNA were detected in 54.0% and 35.5%, respectively.